FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_h-30ar-2.35100000093fff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_h-30ar-2.35100000093fff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12938340
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG532080.41124286423142375Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC445820.3445727968193756TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC192260.14859711524044042Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG121150.028.73103527
CCGAGAG91450.028.51570329
ATGCCGA129750.028.1619726
CGTATGC95450.027.83951845
CTCGTAT95850.027.34838143
GCCGAGA127550.026.9718928
TCGTATG98200.026.92304444
CATCTCG88550.026.70619240
AATGCCG155900.026.6701325
CGAGAGA102350.025.9844830
GGAATGC194200.025.74299423
GAGCGGT228800.025.7146110
ATCTCGT95750.025.54398241
AGAGCGG233600.025.3210669
GCGGTTC234650.025.07352412
GAATGCC188750.025.05599824
AGCGGTT239700.024.5546611
AAGAGCG243750.024.3866658
CAGCAGG238250.024.33580818
TCTCGTA101300.024.29996742
Produced by FastQC (version 0.11.2)