FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_h-30ar-1.35100000093fd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_h-30ar-1.35100000093fd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13793708
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC979260.7099323836636241TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG964350.6991231074342011Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC363950.2638521853587157Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC191080.13852692836472977TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG208350.032.34298327
ATGCCGA218850.031.92212326
CCGAGAG154000.031.90901429
GCCGAGA213400.031.092628
CGTATGC180550.031.0925345
AATGCCG253300.031.01757825
CTCGTAT181150.030.86612143
GGAATGC314750.030.01551423
CGAGAGA167550.029.91935330
ATCTCGT173750.029.8370941
GAGCGGT383450.029.76609210
TCGTATG189350.029.67203144
AGAGCGG388650.029.4604559
GCGGTTC387000.029.4116512
GAATGCC300750.029.27328724
AGCGGTT392550.029.05313711
CAGCAGG387450.028.94777318
CGGTTCA394300.028.88424713
AAGAGCG398150.028.8140288
TCTCGTA180450.028.74151442
Produced by FastQC (version 0.11.2)