FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_ngn2_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_ngn2_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39983350
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2574000.6437679684168535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG153150.026.61729222
GAGTACG228100.025.70698719
GTACGGG227250.025.58769821
AGAGTAC270800.024.85254918
AGTACGG236350.024.73576220
CGCAGAG275900.024.30112615
GCAGTGG297550.022.810542
TCAACGC300000.022.38694811
AGTGGTA307300.022.1525024
CAGAGTA306850.021.92350217
GTGGTAT309200.021.8883485
ACGCAGA308100.021.85297614
AACGCAG312200.021.57815213
GCAGAGT333800.020.2066616
AGCAGTG342600.020.0340161
ATCAACG339950.019.9900710
CAACGCA348650.019.41159412
GGTATCA350750.019.4103187
GTATCAA350800.019.4066528
ACGGGGT107400.019.26589423
Produced by FastQC (version 0.11.2)