FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_ngn2_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_ngn2_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727163
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG636530.6543840172103623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG39950.029.6969922
GTACGGG58350.029.14034521
GAGTACG58850.028.95449419
AGAGTAC72350.028.5121218
AGTACGG61850.027.66033420
CGCAGAG75200.027.53178215
TCAACGC80000.026.01396211
GCAGTGG80250.025.9247462
ACGCAGA80450.025.8232914
CAGAGTA81700.025.6308217
AGTGGTA82800.025.4849034
AACGCAG82650.024.92843213
GTGGTAT84300.024.8212245
ATCAACG87800.023.7430910
GCAGAGT87650.023.65670216
AGCAGTG90650.023.3808041
CAACGCA92900.022.66162512
GGTATCA95650.022.0166637
CAGTGGT98100.021.6953813
GTATCAA96000.021.6836598
Produced by FastQC (version 0.11.2)