FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_lacz2_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_lacz2_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7088189
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380830.5372740484205486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC46350.030.2163618
GAGTACG38950.030.19635419
TACGGGG27650.029.93214422
GTACGGG40350.029.0588621
AGTACGG42000.027.91943220
CGCAGAG50400.027.66055115
CAGAGTA53750.025.80217717
TCAACGC54750.025.46700111
ACGCAGA55750.025.25550314
GCAGTGG56500.025.0170292
AGTGGTA57200.024.5771734
GTGGTAT57950.024.2525755
AACGCAG58650.024.18511813
GCAGAGT59500.023.31132516
ATCAACG63900.022.4150710
CAACGCA64550.022.24219512
AGCAGTG64600.022.157111
GTATCAA64750.021.7538438
GGTATCA65750.021.477627
CAGTGGT67100.021.4217473
Produced by FastQC (version 0.11.2)