FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_lacz1_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_lacz1_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13018093
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG685630.5266746826896996No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG139950.10750422508120044Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA29600.043.8229647
GCCGTAT32800.040.2838148
CGTGTAG35950.036.7557716
GTCGCCG36500.035.8212145
GTAGGGA37300.035.6014219
TCGCCGT36600.035.44111646
TGTAGGG37550.035.18379618
TAGGGAA39000.033.87177320
GTGTAGG39350.033.57721317
GAGTGTA39800.033.27388428
CCGTATC39400.033.18577249
GGTCGCC40550.032.66687444
CGTCGTG42750.031.24310313
GAGCGTC44850.029.55061110
AGTGTAG44350.029.5491729
GCGTCGT46000.028.88632412
CGTATCA46450.028.29685850
AGCGTCG49100.027.20302211
GTAGATC48350.027.17516332
GTCGTGT50000.026.78023114
Produced by FastQC (version 0.11.2)