FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_foxf_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_foxf_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25692955
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2233760.8694056405734568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG127500.025.05077719
TACGGGG86300.025.01571322
GTACGGG128600.024.8065421
AGAGTAC158600.024.1206218
AGTACGG135450.023.52907620
CGCAGAG164100.023.42532715
TCAACGC181400.021.08021511
GCAGTGG187350.020.9564672
CAGAGTA185450.020.94645917
ACGCAGA184400.020.92295314
AGTGGTA188050.020.6996354
AACGCAG186700.020.59238413
GTGGTAT188550.020.5479015
ATCAACG204550.018.82998510
GCAGAGT206350.018.7600516
CAACGCA209900.018.51403412
AGCAGTG215200.018.1902391
GGTATCA218200.017.7527127
GTATCAA218100.017.7443668
GGGTCTA40100.017.45812626
Produced by FastQC (version 0.11.2)