FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n02_foxf1_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n02_foxf1_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579600
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564420.5334984309425688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG33000.026.85687422
GAGTACG51000.023.67199319
GTACGGG50950.023.4881221
AGAGTAC63650.022.4373618
AGTACGG54850.022.13514720
CGCAGAG67300.021.48346915
GCAGTGG71100.020.611392
TCAACGC71250.020.34521911
CAGAGTA73900.019.79366317
AGTGGTA73750.019.5299434
ACGCAGA74150.019.50149514
GTGGTAT72850.019.4820375
AACGCAG75050.019.08441513
GCAGAGT76250.019.00435816
CCGATCT40450.018.2656467
ACGGGGT25150.018.09845223
AGCAGTG83950.017.6289141
CAACGCA84000.017.46246312
ATCAACG82600.017.34157810
GGTATCA83800.017.1803447
Produced by FastQC (version 0.11.2)