FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n01_ngn2_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_ngn2_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39983350
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG153700.026.37124622
GAGTACG222250.025.23782719
GTACGGG225450.024.92513521
AGTACGG234000.024.10298220
GCAGTGG366950.023.2502572
AGAGTAC364100.023.13652818
CGCAGAG372850.022.55641215
AGTGGTA384500.022.2243774
GTGGTAT384750.022.111215
TCAACGC395450.021.37175211
CAGAGTA400200.021.09257117
AACGCAG405250.020.8381113
ACGCAGA406300.020.80134414
AGCAGTG410500.020.7372361
CAGTGGT433000.019.8227653
ATCAACG428850.019.72334910
GTATCAA434600.019.6373638
GCAGAGT432500.019.59709516
GGTATCA439650.019.5296427
CAACGCA439700.019.30726412
Produced by FastQC (version 0.11.2)