FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n01_ngn2_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_ngn2_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727163
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG37550.028.38653622
GTACGGG54150.028.09337821
CGCAGAG87250.027.79402715
GAGTACG55150.027.64652619
AGAGTAC87050.027.58063918
AGTACGG57600.026.53047820
GCAGTGG92050.026.3572942
ACGCAGA93950.025.88534414
CAGAGTA94000.025.72491817
AGTGGTA94500.025.7092864
TCAACGC94050.025.67443311
AACGCAG97550.025.0007913
AGCAGTG97200.024.967211
GTGGTAT97550.024.7638235
GCAGAGT101600.023.90247216
ATCAACG104600.023.2497910
CAACGCA106150.022.9752912
CAGTGGT107250.022.8461193
GGTATCA108300.022.6787877
GTATCAA110100.022.1825758
Produced by FastQC (version 0.11.2)