FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n01_lacz2_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_lacz2_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7088189
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG38750.028.13121
GAGTACG39800.027.38943119
TACGGGG27250.026.96391322
AGAGTAC64300.026.87854418
CGCAGAG65450.026.66980615
AGTACGG41450.026.38199420
GCAGTGG70150.025.241782
AGTGGTA71050.024.9697384
GTGGTAT71550.024.7950735
ACGCAGA71300.024.52999314
TCAACGC71050.024.47012911
CAGAGTA71800.024.35917317
AACGCAG72850.024.05526413
AGCAGTG76250.023.454241
GCAGAGT77000.022.84854116
CAACGCA77100.022.7289412
GGTATCA77850.022.6873727
ATCAACG77500.022.65613710
GTATCAA79850.022.1623258
CAGTGGT80350.022.1226063
Produced by FastQC (version 0.11.2)