FastQCFastQC Report
Thu 29 Jun 2017
HKHMWAFXX_n01_lacz1_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_lacz1_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13018093
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA166670.12802950478230568TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32700.039.56341649
GCCGTCT33700.038.3916452
ACCTATC33150.037.5692339
CCTATCT34200.036.41704640
CGTATGC37500.034.7736947
ACACCTA37850.034.179137
CACGTAC38250.033.9990532
CGTCTGA40050.032.98892217
CTATCTC38900.031.92847441
AGTCACG41400.031.66213229
CACGTCT41750.031.47992915
ATGCCGT41300.031.3249150
TGCCGTC42300.031.15551451
GTATGCC42300.030.66368748
ACGTCTG43300.030.5128516
CACCTAT41350.030.45247838
TCACGTA43150.030.29809431
CGTACAC44650.029.43475334
GCACACG45350.028.9801912
TACGGGG42100.028.35018522
Produced by FastQC (version 0.11.2)