FastQCFastQC Report
Wed 16 Aug 2017
HKHMWAFXX_n01_foxf_ko_10hpf_rnaseq_may_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_foxf_ko_10hpf_rnaseq_may_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25692955
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG84050.027.62108422
GTACGGG124350.025.60457821
GAGTACG125250.025.4483319
AGAGTAC212100.023.6314318
AGTACGG135350.023.57464420
CGCAGAG216050.023.26306715
GCAGTGG230650.022.2358882
GTGGTAT233750.021.7627535
TCAACGC232500.021.70603411
ACGCAGA235200.021.51569214
AGTGGTA237700.021.4737034
AACGCAG241600.021.14563213
CAGAGTA239150.021.073917
GAGTACT111850.020.2932520
ATCAACG255950.019.86559110
GCAGAGT256050.019.75032616
AGCAGTG264250.019.5436781
GGTATCA265900.019.2783097
CAACGCA267350.019.01850712
CAGTGGT270800.018.9638123
Produced by FastQC (version 0.11.2)