FastQCFastQC Report
Wed 16 Aug 2017
HKHMWAFXX_n01_foxf1_ko_10hpf_rnaseq_june_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHMWAFXX_n01_foxf1_ko_10hpf_rnaseq_june_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579600
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG34800.026.2686522
GAGTACG51650.025.64721519
GTACGGG52050.025.25081321
AGTACGG55950.023.79909120
GCAGTGG89250.021.8065912
AGAGTAC89250.021.60642618
CGCAGAG91300.021.12108615
GTGGTAT92650.020.6332135
ACGCAGA95500.020.37280814
AGTGGTA95200.020.3706054
TCAACGC96000.020.08665311
GAGTACT46450.019.97743620
GGGTCTA16700.019.83014526
CAGAGTA99400.019.60836417
AACGCAG99400.019.50397313
AGCAGTG105350.018.6096171
GCAGAGT105550.018.46585816
ATCAACG106250.018.34368310
ACGGGGT26650.018.25109523
GGTATCA107150.018.1580987
Produced by FastQC (version 0.11.2)