FastQCFastQC Report
Thu 12 Mar 2020
HKGG3DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74164455
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5385230.7261200800302516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC123400.032.1788861
GATCCCG142300.029.3816761
GATCCGG146400.028.6083221
GATCCTG227900.027.4710371
GATCACG183150.027.417761
GATCCAG272150.027.2911071
GATCCAC295750.026.2403931
GATCCGA256050.025.7526681
GATCGCG91600.025.3139651
GATCGTG229550.024.9060781
GATCCGT193600.024.7775441
GATCTGC214200.024.2552281
GATCAAC477600.024.1233961
GATCCTT484700.023.829831
GATCAGC249550.023.258441
GATCGCA183050.023.0783331
GATCGCC126450.022.5778731
GATCCCA231650.022.2404171
GATCCTC334600.022.0242251
GATCGGC180050.022.0140441
Produced by FastQC (version 0.11.5)