FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n02_DJ58-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n02_DJ58-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505619589
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC1393750.030.0137351
GATCACG2139000.027.1827031
GATCCGG1613250.026.6715431
GATCGCG966750.026.0297221
GATCCCG1670700.025.42461
GATCCTG2741550.024.8709131
GATCTGC2663350.024.4578881
GATCCGA3094600.024.3082981
GATCCAG3231100.024.0599651
GATCCTT6524300.023.6631641
GATCAGC2960000.022.9594961
GATCCTC4061700.022.7882041
GATCGTG2705250.022.6855141
GATCCGT2388650.022.6481021
GATCCAC3540200.022.4077381
GATCTCG2760900.022.167861
GATCTTG4278450.022.0065251
GATCAAC5538700.021.9339831
GATCTGG2602350.020.8969331
GATCACC2569300.020.6832241
Produced by FastQC (version 0.11.5)