FastQCFastQC Report
Thu 12 Mar 2020
HKGG3DRXX_n02_DJ53-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n02_DJ53-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229792542
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC769800.035.345191
GATCCGG903900.031.882061
GATCACG1159350.031.2856771
GATCGCG510650.031.0637321
GATCCAG1767400.029.2307931
GATCCGA1664200.029.1745641
GATCCCG885050.029.1370561
GATCCTG1483100.028.953491
GATCCAC1919200.028.777321
GATCTGC1385550.027.7792241
GATCGTG1417500.027.096831
GATCCGT1240100.026.8106271
GATCAGC1568600.026.6543061
GATCAAC2960600.026.184811
GATCCTC2097750.026.0203511
GATCTCG1459500.025.8650361
GATCCTT3318650.025.6622731
GATCACC1378400.025.4828591
GATCGCC811650.025.0817551
GATCCCA1514100.024.6929051
Produced by FastQC (version 0.11.5)