FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n02_DJ52-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n02_DJ52-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696356461
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC2029450.034.1799961
GATCGCG1338650.033.3992041
GATCACG2892950.032.831651
GATCCGG2375200.030.8741421
GATCAAC7312550.030.0243471
GATCCAG4497250.029.8441331
GATCGTG3448900.029.7822271
GATCCAC4702300.029.4431321
GATCCTG3717650.028.8617361
GATCCCG2332400.028.3043921
GATCGCC2188650.027.642621
GATCAGC4062200.026.911661
GATCTGC3595550.026.436241
GATCCGA4050400.025.9071621
GATCATG5334000.025.5837971
GATCACC3514950.025.5037571
GATCGCA2870600.025.4777811
GATCGGC2986150.024.6327231
GATCCCA3769900.024.3693831
GATCGCT2474300.024.2257561