FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n02_DJ51-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n02_DJ51-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562722817
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC1480200.034.8631321
GATCCGG1791150.031.0733741
GATCGCG1050100.030.9711591
GATCACG2301950.029.371651
GATCCCG1713150.027.8669341
GATCCAG3448250.027.079831
GATCGTG2851900.026.9133321
GATCCTG2943900.026.6831
GATCTGC2711650.026.1423851
GATCCAC3666500.026.1263391
GATCAGC3128950.025.7305321
GATCCGA3278300.025.4760781
GATCAAC5992400.025.2251681
GATCGCC1624650.025.0564041
GATCCGT2459200.024.5749511
GATCGCA2361300.023.9051821
GATCGGC2245350.023.8836351
GATCCTC3965300.023.5652471
GATCACC2751450.023.1056021
GATCTTG4572900.022.8914991