FastQCFastQC Report
Thu 12 Mar 2020
HKGG3DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74164455
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC127100.033.843171
GATCCGG141500.030.859651
GATCCCG142400.030.1052231
GATCCAG277450.028.8669261
GATCACG184700.028.8563291
GATCCGA261400.028.2846151
GATCTGC222000.028.2484781
GATCCAC306950.027.7912311
GATCCTG233650.027.0879271
GATCGTG224550.026.6699731
GATCAAC480400.026.590451
GATCCGT191950.024.8237781
GATCAGC255200.024.8005261
GATCGCA186150.024.7802981
GATCTGG222300.024.5293271
GATCCTT506950.024.355071
GATCCCA240900.024.2586611
GATCACC225300.024.2027051
GATCGCC132700.023.6290951
GATCCTC339250.023.4802651
Produced by FastQC (version 0.11.5)