FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n01_DJ58-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n01_DJ58-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505619589
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC1373650.030.8602771
GATCACG2108600.028.0650791
GATCCGG1588100.027.4373051
GATCGCG924750.027.013571
GATCCCG1695250.026.0068021
GATCCTG2734100.025.9648271
GATCCGA3041150.024.8788361
GATCCAG3197500.024.7486041
GATCTGC2627450.024.5901051
GATCCTT6564550.024.3170621
GATCCGT2331100.023.3861391
GATCCAC3519850.023.295861
GATCAGC2911650.023.273261
GATCTCG2700000.023.2310831
GATCGTG2618400.023.2073291
GATCCTC4054950.022.8879891
GATCAAC5493600.022.498681
GATCTTG4227350.022.3490981
GATCCCA2918850.021.2631871
GATCTGG2545050.021.1324121