FastQCFastQC Report
Thu 12 Mar 2020
HKGG3DRXX_n01_DJ53-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n01_DJ53-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229792542
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC759800.036.5235181
GATCCGG854150.033.4568981
GATCACG1118100.032.459131
GATCCCG881450.030.8494191
GATCCAG1699000.030.7506331
GATCCTG1429150.030.5292151
GATCGCG529400.030.2432561
GATCCAC1890050.029.6871721
GATCTGC1381050.029.14051
GATCCGA1619350.029.074891
GATCCGT1204050.027.9318471
GATCGTG1332350.027.789191
GATCAGC1520550.027.3397561
GATCAAC2898350.027.1751771
GATCCTC2137000.026.6433731
GATCCTT3364900.025.8510231
GATCGCC800800.025.3902721
GATCCCA1499550.025.2758031
GATCACC1371050.025.2330381
GATCTTG2220300.025.0689831
Produced by FastQC (version 0.11.5)