FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n01_DJ52-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n01_DJ52-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696356461
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTCGTAT15397860.22112037242948768TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10520900.037.336251
GATCCGC2017750.034.5971531
GATCGCG1308600.034.1453481
GATCACG2853450.033.315721
GATCCGG2342300.031.5060561
GATCAAC7327250.030.9419781
GATCGTG3359100.030.6752361
GATCCAG4454000.030.3777031
GATCCAC4744400.029.9565331
TCGGAAG11172450.029.1309073
GATCCTG3664600.029.0123841
GATCCCG2325700.028.9435231
AGAGCAC10966550.028.5436748
GATCGCC2188650.028.0590271
CGGAAGA11312750.028.049244
GATCAGC4036000.027.2858561
GATCTGC3581600.026.9737721
GATCCGA4037200.026.3006531
GATCATG5318800.026.21561
GATCACC3521700.025.8676971
Produced by FastQC (version 0.11.5)