FastQCFastQC Report
Fri 13 Mar 2020
HKGG3DRXX_n01_DJ51-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKGG3DRXX_n01_DJ51-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562722817
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCTCGTAT7101060.1261910799682395TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC1483900.034.0455781
GATCGCG1005450.031.0983811
GATCCGG1703450.030.6537021
GATCACG2254850.029.4801081
GATCCCG1742750.027.7946281
GATCCAG3373400.027.3597321
GATCGTG2720200.027.0706461
GATCCTG2908850.026.8007561
GATCTGC2695800.026.7373431
GATCCAC3726300.026.484991
GATCAGC3101100.025.8150391
GATCCGA3248850.025.4266911
GATCGCC1642750.025.306381
GATCAAC6023800.025.2457831
GATCGGA8426200.025.04871
GATCCGT2406150.024.2661341
GATCCTC4037350.024.2110161
GATCGCA2335150.023.7587151
GATCGCT1997450.023.5570771
GATCGGC2200400.023.3385451
Produced by FastQC (version 0.11.5)