FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n02_CIVR_Y4_D28_P2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n02_CIVR_Y4_D28_P2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255977
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTACCCAAGTGTAGATC187127.310031760665997Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60932.3802919793575206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG14170.5535653593877575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT11220.4383206303691347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG7250.2832285713169542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG6000.2343960590209277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT4640.18126628564285072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG3850.1504041378717619No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG3840.15001347777339372Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG3710.14493489649460692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTG3290.12852717236314198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG3200.12501123147782808No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG3130.1222766107892506Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT3030.11837000980556847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT2940.11485406892025454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)