FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n02_CIVR_Y4_D28_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n02_CIVR_Y4_D28_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76938
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTAGTGTAGATC790210.270607502144584Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18742.435727468871039No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG2730.3548311627544256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT2190.2846447789128909No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT1980.2573500740856274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG1610.2092594036756869No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTG1370.17806545530167148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG1320.17156671605708493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT1290.167667472510333No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1190.1546699940211599Illumina Single End PCR Primer 1 (100% over 32bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGGTGCTAGTGTAGATC1180.15337024617224257Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT1070.13907301983415218No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTAGTGTATATC1000.129974784891731Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT1000.129974784891731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG960.12477579349606176No Hit
CCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCT910.11827705425147522No Hit
GATCGGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTA910.11827705425147522Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGG870.11307806285580599No Hit
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC870.11307806285580599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGG810.10527957576230212No Hit
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTA790.1026800800644675Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTG790.1026800800644675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG780.10138033221555019No Hit
GGAGTTCAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG780.10138033221555019Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA770.10008058436663286Illumina Single End PCR Primer 1 (100% over 34bp)
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA770.10008058436663286Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)