FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n02_CIVR_Y4_D28_P1_C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n02_CIVR_Y4_D28_P1_C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29675457
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC2004600.6755077099570868No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1195010.4026930402453448No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1082450.36476270609750006No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC824840.2779535964686239No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT660060.22242622919000035No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG650880.21933276377175928No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC577310.19454123318134578No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA532560.17946143171442988No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC529580.17845723487931459No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG527950.17790795942923474No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC526860.17754065253316906No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG507780.17111109695800136No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA502840.16944642166757531No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT494100.16650122692297545No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT476040.1604153897276123No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA458150.15438683892888322No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC456930.15397572478833266No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT451390.15210886221566866No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC421400.14200286789180702No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC416930.14049657263913407No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC411870.13879145989226047No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG407300.1372514667592145No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC399620.13466346954656838No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG393310.13253713329503233No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG391560.13194742038850488No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA389020.13109149422703079No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT387240.13049167195639144No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA377880.127337550353479No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT372140.12540329202006897No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC365100.12303096124181004No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT363980.12265354498163246No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC363510.1224951649438794No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT359080.12100234884335563No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC348660.11749102970848942No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG345770.1165171609657098No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA344190.11598473445581646No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT340550.1147581316102394No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT339860.11452561623566573No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA334630.112763217092158No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA332020.1118837024144228No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG331180.11160064021928963No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT327810.11046502165071966No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC325720.10976073595092403No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA323220.1089182889415991No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA320020.10783995676966322No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA319780.10775908185676805No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG314680.10604048995774523No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC314490.10597646398503653No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG311090.10483073605235464No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA310800.10473301219927295No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG308030.10379958091294096No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA306480.10327726376715951No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG302390.10189902045990396No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC297090.10011303280013514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)