FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255977
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTAT228808.93830305066471TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGTAT124834.8766100079304TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTA83943.279200865702778TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGTA56392.2029322946983516TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTTT29741.161823132547065TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTT17450.6817018716525314TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGTTT13430.5246565121085097TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGTT12220.477386640205956TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGGAT6320.24689718216871046TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGGAT5440.21251909351230774TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCGCGGA4540.1773596846591686TruSeq Adapter, Index 27 (97% over 38bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3870.15118545806849834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGGA3510.13712169452724268TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTGT3290.12852717236314198TruSeq Adapter, Index 27 (97% over 38bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3060.11954199010067311Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCAACATCTCGTG2720.10625954675615387TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)