FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76938
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTAT1395318.135381735943227TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTAT831910.812602355143103TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTA45255.881359016350828TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTA33004.289167901427123TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTTT3450.448413007876472TruSeq Adapter, Index 25 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTATG2450.31843822298474095TruSeq Adapter, Index 25 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTA2200.2859445267618082TruSeq Adapter, Index 3 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTT2020.26254906548129664TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTTT1810.23525436065403313TruSeq Adapter, Index 25 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTATG1760.22875562140944658TruSeq Adapter, Index 25 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTA1510.1962619251865138TruSeq Adapter, Index 3 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTT1480.1923626816397619TruSeq Adapter, Index 25 (97% over 37bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1250.16246848111466378Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAA940.12217629779822715TruSeq Adapter, Index 25 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAA900.11697730640255792TruSeq Adapter, Index 25 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC860.11177831500688866Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG830.10787907146013673No Hit
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG810.10527957576230212No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGA810.10527957576230212Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC780.10138033221555019Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)