FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P1_H8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P1_H8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29810243
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC2414700.8100235882008745No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2281130.7652168417412767No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA2281030.7651832962247238No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1574080.5280332669545833No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1372610.4604491147556227No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG1258820.42227767147017214No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1251460.41980872145188486No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG1247930.4186245647175704No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1161380.38959092014110724No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC1125080.3774138976324346No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC1092480.36647805923621624No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA1074780.36054050280636757No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT1066260.3576824247960676No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC928830.311580821397531No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC901550.3024296044819225No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC886010.2972166312096148No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC859750.288407578562845No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT848670.28469073532879285No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC838170.2811684560907471No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC826980.27741471278848684No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA822580.2759387100601629No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT812340.2725036491651544No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC785240.263412814179341No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA783760.2629163405343593No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC773420.259447734122798No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA746820.2505246267197486No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA739430.24804561304649547No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA729940.24486214352563312No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG729270.24463738856472925No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA719080.24121910042799719No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA695320.23324868569504784No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG687280.23055162616420133No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC684560.22963918811396475No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC668990.2244161511866911No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA665600.2232789581755506No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG662410.22220885619751574No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT661210.22180630999888196No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT651640.2185960040647773No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA628240.21074635319141816No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC626160.21004860644711953No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT615980.20663367286204276No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG612490.20546293433434942No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG607270.20371185837029238No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC591630.19846533958143178No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG590740.1981667844841117No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC588410.1973851739484311No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT584530.1960836079061818No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG583640.19578505280886171No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA581170.1949564785500071No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG574740.192799501835661No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC573090.1922460008125395No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG572750.1921319460562599No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG572260.1919675730251511No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC570450.19136039917554512No Hit
CGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCG568940.19085386187559758No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG559630.18773077428453033No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT553920.18581532528936445No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG550480.18466135951994755No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC549720.18440641359414614No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA529050.17747255532267886No Hit
CGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGT526590.17664733561547954No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG524970.17610389824732392No Hit
CTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCA516530.17327265665026617No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG514710.17266212824900487No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA509790.17101168883460627No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG506340.16985436851353408No Hit
GGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACG501640.16827772923555168No Hit
CTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCC499580.16758669159456366No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT497160.16677489009398547No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA496170.1664427894801126No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT486760.1632861563724925No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC482250.16177325357596045No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG482120.1617296444044418No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT481590.16155185316671186No Hit
GTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCG474180.1590661303901481No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT471410.1581369195816351No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA461890.15494338640580688No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG457480.15346402912582766No Hit
GCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGC452700.151860553434603No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC451390.1514211071677611No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT451200.15135737068631075No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG450550.15113932482871742No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG447200.15001555002419806No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG441610.14814035564889558No Hit
TCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAG433090.14528227763859555No Hit
CCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAA432520.14509106819424453No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA430500.1444134487598776No Hit
CGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCC429870.14420211200559485No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT427990.14357145629440188No Hit
ACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAG427500.1434070832632931No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA423540.14207868080780153No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA418450.14037121401526315No Hit
TGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAG418340.14033431394705503No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC417100.1399183495418001No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT412070.13823101005919342No Hit
AGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTC408190.13692944401694412No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA405490.13602371507001804No Hit
GCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAG405240.135939851278636No Hit
GCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACG404950.13584256928063282No Hit
GCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTC398070.13353463774179902No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG394560.13235719011079514No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA394390.13230016273265535No Hit
CCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCG394070.13219281707968633No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG392170.1315554522651828No Hit
CTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAA388480.13031762270438388No Hit
GGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGG386700.12972051250974373No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG386570.12967690333822504No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG386250.12956955768525605No Hit
GCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGC386150.12953601216870322No Hit
GCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCC385360.12927100258793597No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG385270.12924081162303844No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC384620.1290227657654451No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT384130.12885839273433633No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA382490.12830824626287013No Hit
AGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTC381310.12791240916754687No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGC378810.12707377125372646No Hit
GCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACC376220.12620494237500848No Hit
GTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGG375620.1260036692756916No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCT375400.1259298691392754No Hit
TGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC375360.12591645093265427No Hit
TGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCAC370680.12434652075798243No Hit
CAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACC370460.12427272062156623No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT363710.12200839825425107No Hit
GCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAG358960.12041498621799225No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA356640.1196367302339669No Hit
CAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCG351620.11795274530301547No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG351530.11792255433811794No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG350990.11774140854873275No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT350140.1174562716580338No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA348700.11697321621967322No Hit
TGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTG348200.11680548863690914No Hit
GGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGG348000.1167383976038035No Hit
GGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTA347520.11657737912434997No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG346880.11636268781841194No Hit
CGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGC339790.11398431069481721No Hit
GTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACG337010.1130517453346489No Hit
GGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG336890.11301149071478551No Hit
CCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGC335860.1126659718942915No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA332980.11169986101757037No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG332260.11145833329839008No Hit
CCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTG330510.1108712867587158No Hit
GCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCT328890.11032784939056016No Hit
GACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGG328620.11023727649586755No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC326240.10943889320191051No Hit
CTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGG324540.10886861942051261No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC323900.10865392811457458No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTC322540.10819770908945628No Hit
CGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTG318990.10700684325183125No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG317970.10666467898299253No Hit
GTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGG315830.10594680492876224No Hit
CCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCC308130.10336380015419531No Hit
CATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTA307980.10331348187936609No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA306200.10271637168472594No Hit
GCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGA302080.10133429640274988No Hit
GCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTC299390.10043192200747911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)