FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P1_H2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P1_H2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10324082
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGT654610.6340612172588322TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGT436020.4223329493121035TruSeq Adapter, Index 2 (97% over 36bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC293680.28446112690697345No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGTT248060.2402731787678556TruSeq Adapter, Index 2 (97% over 36bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA243770.23611784563508892No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC227250.22011642294201073No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGG201370.1950488188683507TruSeq Adapter, Index 2 (97% over 36bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC145690.14111666296335112No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGTT132190.12804043981828117TruSeq Adapter, Index 2 (97% over 36bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT125330.12139578124234195No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT123050.1191873524445079No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG117420.1137340830884528No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG116400.11274610178415863No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGG113750.1101792876112375TruSeq Adapter, Index 2 (97% over 36bp)
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT112470.10893946793526048No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC111390.10789337008365489No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC103510.10026073020342147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)