FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P1_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P1_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326923
Sequences flagged as poor quality0
Sequence length151
%GC78

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTTT6762720.685910749626057TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT4096412.530167654157095TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGTT3847611.769132180972278TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTTT239507.325884076678606TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGTT234677.1781428654453805TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT165415.059601190494398TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGAT163475.000260000061176TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTGT147694.517577533547655TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGAT113353.4671772863946555TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTGT68432.093153433683161TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGGT65091.9909887037620482TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGGT52941.619341557492131TruSeq Adapter, Index 23 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38051.1638826267959121Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTT15670.4793177598394729TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAAC12980.39703538753773826TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGT12780.39091773903946797TruSeq Adapter, Index 23 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAAC12420.37990597174258156TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTA7600.23247064293426892TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGT7520.23002358353496083TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTT6100.18658827919724216TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAA4960.15171768275710182TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTT4950.15141180033218832TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGTTG4630.14162356273495594TruSeq Adapter, Index 23 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGT4620.1413176803100424TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGTG4400.13458826696194517TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTTTG3980.12174120511557768TruSeq Adapter, Index 23 (97% over 36bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA3930.12021179299101012No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGA3820.11684708631696147TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTCT3570.10920002569412368TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTG3510.10736473114464262TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTTA3270.10002355294671833TruSeq Adapter, Index 23 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)