FastQCFastQC Report
Sat 29 Apr 2023
HKG7TDMXY_n01_CIVR_Y4_D28_P1_C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7TDMXY_n01_CIVR_Y4_D28_P1_C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36634018
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC2563690.6998113065293574No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1986770.5423292634730921No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1933750.5278563765514337No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1468670.4009033352552265No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1070090.29210282093544854No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG977490.26682576833368377No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC874050.2385897173495957No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA819650.223740131371885No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA813960.2221869301914958No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG806720.22021062499887398No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC786810.21477578571916406No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC767340.20946105338486212No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC759790.20740012738979385No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT725830.19813005496694355No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT710930.19406279704290147No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC676320.184615293905244No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC666780.18201115695253522No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG653660.17842978621673442No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC643990.17579016312106413No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC640810.1749221174701612No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT628460.17155093388882434No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA616760.16835718102229463No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT590590.1612135474738261No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT588570.1606621474062714No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA585440.1598077502718921No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG583790.15935734922661227No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC574170.15673137464746564No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT569860.15555487252312863No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA565080.15425007434346952No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA559560.15274327811926064No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC546490.14917555589998344No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG540710.1475977873898517No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA529580.1445596276116914No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA525420.1434240710369253No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG520530.14208924612091417No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG519830.14189816688958334No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT514010.1403094795662327No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA509450.1390647348592775No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC495470.13524860963927027No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG493260.13464534520892577No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT491280.13410486395458995No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA475810.1298820129421785No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT475000.12966090697449567No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC472390.12894845441196212No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA467050.12749079284723833No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA465590.12709225616474829No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA461040.12585024116109786No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG459200.12534797575302825No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG458090.12504497868620362No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG457060.12476381924581682No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC449340.12265648829456817No Hit
CGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGT442970.12091766728945758No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA439890.12007691867160189No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA439290.11991313647331833No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC437470.11941633047185815No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC436510.11915427895460443No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT435270.11881579574481839No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG430040.11738816091644658No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG428530.11697597571743291No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC421600.11508429132725763No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT419700.11456564769935965No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG414630.11318168812386345No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG405080.11057482146784992No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG402890.10997701644411488No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC401880.10970131641033752No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT398530.10878686580325424No Hit
CTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCC391670.10691428933621203No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT389700.10637653778518097No Hit
GGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACG388480.10604351398200437No Hit
CGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCG385530.10523825150711014No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG383280.10462406826354674No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC380980.10399623650345971No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA377740.10311181263272841No Hit
GCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGC375840.10259316900483043No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG370990.10126926290203822No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA367900.1004257845808778No Hit
GCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACG366400.10001632908516887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)