FastQCFastQC Report
Tue 28 Sep 2021
HKG7MDRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKG7MDRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048362792
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG20473390.1952891704687665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGCA14322860.13662121652253376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11735770.11194378596374299No Hit

[OK]Adapter Content

Adapter graph