FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252995
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74130.14111949468826832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21250.014.1240981
GTATCCT20850.013.8986831
TAGGTAG10450.013.8650365
CGTCCGG6550.013.69602332
CCTCCTA10300.013.3974821
GCTTAGG14200.012.38854169
GACGCGC5900.012.28147238
AGTCCGG7950.012.14937910
CGCACCT8900.011.6283866
TAATACT35100.011.4008924
GGCTCGG8000.011.2116973
GTATATA46800.010.9097861
TCCGCAC9500.010.8940664
GGTAGTC13200.010.7147327
CTAATAC24800.010.7109263
CGCGCAT6807.2759576E-1210.65628740
GTATTAT40800.010.5693981
GTCTTAG13150.010.4938451
CCCCTAG4956.564733E-810.4540961
ACGCGCA6951.0913936E-1110.42629639
Produced by FastQC (version 0.11.2)