FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996080
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64210.12852076027605644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20100.014.7619521
CGTCCGG7300.013.23408632
GGCTCGG5950.012.7564473
CCGTCCG7700.012.54647631
CTAATAC20600.012.0583893
GTATAGA21900.011.6581331
TAGGTAG11350.011.5509075
GTATATA47750.011.4885471
GGTAGTC10900.011.3938267
CGACGTA9600.011.14153345
GTATTAT40550.010.9759031
GTATAAT31500.010.9529441
CGGGGCA3501.5989479E-510.8416789
TATTAGG19200.010.6018942
GTATCCT17250.010.6005451
TATTATA59600.010.5934532
GTCCTAG7900.010.4815511
TCGGTCG4956.5581844E-810.4547684
TATACGG15750.010.2954575
CTATGCA17300.010.1694149
Produced by FastQC (version 0.11.2)