FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740415
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201930.7368591983330992No Hit
ATATACATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCG31580.11523802051879005No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATCAAAATCAGCCATTTCCGCACCTTTT28920.1055314614757254No Hit
CATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCGGTATG28290.10323253959710482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7950.023.4338255
CGCATAG7400.023.31067720
TAGTCAG7550.023.3019499
CTACGGA7800.022.9973479
TGGGCTC5550.022.3786563
ACACTCG7750.021.8119815
GCGACCG5250.021.68400448
CCGGTTA7050.021.53336132
GGCTCGG7750.021.3680081
CCGCACG8850.021.0495763
ACATAGG8250.020.91011631
CGACCGG5450.020.88749949
CGCGCGC9250.020.88598468
CCTACGG8450.020.8193238
CGCACCT10750.020.5390466
CACGCGC9250.020.5130266
AGCCGAT9250.020.5103998
AATCCGG7450.020.37795329
GGAAACC9200.020.24914413
ACAGGTC9450.020.08071332
Produced by FastQC (version 0.11.2)