FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7598351
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88470.1164331576680256No Hit
ATATACATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCG84160.11076087430022645No Hit
TCTTACAACTCCGCATACCGCGTCGCCGCGTCGCCGCGTCGCCAAAAATTTACTTCGCCAACCATTCCATATCTG78940.10389096265755557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG15650.018.077235
CCGACGC12550.017.3157269
GTATTAG22350.016.9802721
GCGGAGT42300.016.47318660
TATGCGG46000.016.19836257
GGTATGC48650.015.59955255
GTATGCG49750.015.04671956
TAGTCAG12650.014.9976929
ACTTCGC53450.014.90849252
ACGCGGT51650.014.62678851
TGCGGAG49750.014.56114959
CTATGCA22650.014.4679739
CGCGGTA56200.014.36312752
TATTAGG21650.014.3421442
GCGGTAT53700.014.196753
CTTCGCC56150.014.19160953
CCTAATA25050.014.048242
ACATGTA64550.014.0035265
CGCCAAC55850.013.95919756
ACTCCGC64050.013.949048
Produced by FastQC (version 0.11.2)