FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392201
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49020.14450794631568117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13150.016.5281931
TAGGTAG7750.014.6893835
CCCCTAG2506.117647E-613.7997291
TATTAGG13250.013.7991172
CTATGCA11600.012.4900289
CGCACCT7000.011.82781566
GTATATG20750.011.8045871
ACATAGG7400.011.65637931
GCCCGAG4207.669587E-811.49909560
GTATTAT27400.011.3318941
ACTATGC13600.011.1605578
GTCCTAA8400.011.0890671
TCCTAAG7800.011.0569862
TAATACT24150.010.9991354
TCCGCAC7101.8189894E-1210.68977464
GGCTCGG4854.8619768E-810.6698931
GTATTAC14050.010.5585121
GTATAAT21950.010.5305441
GGTAGTC8850.010.5242857
GTATAAA25850.010.4098541
Produced by FastQC (version 0.11.2)