FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5634091
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110690.1964647003394159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24750.014.4964891
CGCGCGG4900.014.07978954
GTATTAT47800.013.424241
CGCGGGA4406.184564E-1113.32839356
TCCTTGC30850.012.5238158
CCTCCTA10600.012.3675141
GGCTCGG6900.011.9996025
TAGGTAG13300.011.9319345
GCGCACT8350.011.567245511
TATTAGG24050.011.4757112
GTATATA57950.011.31111
TTATACT36750.011.1710584
TACACAG18600.011.1286635
TCCTATA22650.011.1188362
TATACTG36100.010.8943765
CCTATAC19350.010.6972253
CGACCCG3551.8678766E-510.69045121
CGCGCAT6201.4915713E-1010.57438940
GTATCCT22100.010.4589291
GTCCTAT14550.010.4326441
Produced by FastQC (version 0.11.2)