FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499563
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92670.1425788164527369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT12150.015.33194466
GTATTAG30600.014.5436951
CCGCACC12300.014.0231265
TCCGCAC12450.013.85448664
GGCTCGG7050.013.212285
CTAATAC35250.013.2121793
TAGGTAG14700.012.4383175
TCTATAC30500.011.8765223
CCCTTAG21000.011.8282291
CGCGCGG4401.15851435E-811.76047954
GTATAGA29450.011.5972861
CCTACAC11650.011.5488433
GTATATA77100.011.4996661
CTATACT29200.011.4601074
TATACAG42250.011.3498795
CCTATAC22300.011.29322053
CGAATAG28650.011.19733569
CTTAGAC20850.011.0858393
TCCTATA28500.011.0153852
ACTATGC30750.010.9937038
Produced by FastQC (version 0.11.2)