FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357916
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44291.2374411873176945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC200.007352851351.75366641
CCCGGAC501.8580887E-434.5024525
CGTTCGG553.2538944E-431.36147512
CGAGCGT450.004620036630.6645557
CCGAGCG500.007719971227.6058166
ACTGCGC500.00773051327.59818
CAGGACC951.0464195E-525.4264094
TAAGTGG851.43853E-424.3580725
CGCGTGT1253.4983968E-622.07539469
CTAATAC1906.675691E-1021.7940643
TACACAG1154.5838115E-521.0044235
ACGCGTG1701.0820622E-720.28988569
TACGCGT2900.020.21992169
CTATACT1409.296966E-619.7184414
ACACAGT1056.0033443E-419.718446
AGACCGT1258.7054876E-519.324076
TCCACAC900.005719891719.1707063
ACCGTCG900.00572914119.1653468
CTGTGGG900.00572914119.16534611
CGTGTAG900.00573377119.1626769
Produced by FastQC (version 0.11.2)