FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104085
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3580.3439496565307201No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTGC252.5563105E-455.1999978
TTAGGTA200.007340329251.7500044
GTCGGGA200.007340329251.75000411
ACACAGA306.284881E-446.0000046
GTATTAG400.002585536834.51
TACACAG400.002585536834.55
GTTCGTG450.00460358330.66666629
CCACACC450.00460358330.6666664
GTATAAC500.00770314627.5999981
ATATAAC700.00132571224.6428593
TTTACAC750.001980929623.03
ATTCTGA900.005700154719.1666661
ACGCTGC2601.3228158E-411.94230769
CTGACGC3209.023281E-49.70312566
GACGCTG3000.0058044219.20000168
TCTGACG3450.00179387369.065
CATCTGA4050.00762757247.66666763
ATCTGAC4050.00762757247.66666764
TACACAT6057.4999727E-46.84297569
ATACACA6550.00184418766.32061168
Produced by FastQC (version 0.11.2)