FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919411
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65510.1331663485730304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21400.015.4763111
TAGGTAG11500.014.6996935
GTATAGA22300.012.3764231
TATTAGG21850.011.8418582
GTATTAT42100.011.554391
GTATATA52700.011.2597271
GGCTCGG6951.8189894E-1210.9206343
GTATAGG16500.010.87251
TAATACT36500.010.7751164
TAGGACT7700.010.7530224
TAGTCAG10950.010.71036159
GGTAGTC12000.010.6363037
CTAATAC23900.010.3930873
TATACAG28400.010.3254885
TATACTG29950.010.0214935
GTATCCC10400.09.95171451
TCCTTGC28200.09.7855378
TTAGGTA20100.09.7833774
ACATAGG11350.09.72805331
ATAGGAG13200.09.67133433
Produced by FastQC (version 0.11.2)