FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149057
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63480.12328471019062326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10750.016.3671955
GTATTAG21800.014.0850611
CGCGCGG3952.3301254E-913.09992354
GGTAGTC12050.012.3097027
CTCCTAG7800.011.5001611
TAGTCAG11700.011.497489
GTATATG29350.011.40220451
CTAGACT10600.011.3913374
GTATATA48750.011.1816951
GTATTAT42000.011.0894421
TAGGACT8400.011.0891194
ACATAGG12450.011.08621731
TATACAG26450.010.9563575
CTATACT21350.010.8265354
GTATCCT21500.010.7513141
ACGCGCG5502.5083864E-910.66264653
GTATAGA24000.010.637651
CGCACCT9500.010.53090966
TATTATA55450.010.4526042
GTTCCTA14950.010.3847611
Produced by FastQC (version 0.11.2)