FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5079365
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72070.1418878147169971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22100.015.1430141
CGCACCT8550.014.5256366
TAGGTAG12400.014.4669185
GGCTCGG8500.013.7992145
TCCGCAC9650.013.58485364
CCGCACC9700.013.51482765
TATTAGG24800.013.3548682
GGTAGTC12300.012.9011967
CTCGGAG10050.012.7002697
GCTCGGA10000.012.0739566
TAGTCAG13050.011.6303769
GGGCTCG9700.011.3810194
CTAATAC24300.011.3578243
TAATACT39100.011.1172334
GTATATG31150.011.0757931
GTATTAT41900.010.869081
TACACCG9700.010.6694955
TATACGG18150.010.6440225
CGCGCAT5201.1859811E-810.61718640
ACATAGG13450.010.51768331
Produced by FastQC (version 0.11.2)