FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679019
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44060.1197601860713413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16500.015.4726851
ACCGTGC4253.45608E-1113.7990258
GGCTCGG6000.013.2247843
GGGCTCG6700.012.8730982
GCTCGGA6550.012.6410144
GTATAGA17150.012.2711031
TATACGG13650.012.1318355
CGTGGGC5301.3569661E-911.0660081
GTATATG24650.010.9168061
CTCGGAG7600.010.8947075
CTATACT16850.010.8514544
TATACTG23550.010.84073455
GTGTAGG6402.3646862E-1110.781221
GTATTAT35000.010.7442561
CTTAGAC10300.010.7182713
GTCCTAT9350.010.7005051
CCTACAC7450.010.6508333
CCCCGGC3258.9946254E-510.61506714
GTCTTAG10100.010.589081
TAGAGCG4905.6521458E-810.5610517
Produced by FastQC (version 0.11.2)