FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447512
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67960.12475419971539302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG8750.014.9825775
GCTCGGA9900.014.6359566
CTCGGAG10850.013.9896237
GTATTAG23100.013.291961
GTATATA63450.012.4513041
CTAGGGT8400.011.9104924
AGCGCAC11300.011.90576510
GTCTTAG12250.011.828351
GTATAAT38900.011.7954111
TAGGTAG12400.011.6852665
GGGCTCG10300.011.3881374
GTACTAG17650.011.3368991
GTATAGA26450.011.086751
GTCCTAA12450.011.0841311
GTATTAT48750.011.0397941
TCCTTGC29700.010.9180868
GTGCGTG11400.010.89543432
CCTTGCG15600.010.8352429
GAGCGCA9300.010.7567779
CGTGGGC8700.010.70669651
Produced by FastQC (version 0.11.2)