FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401867
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65960.12210593115306245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27650.016.7199971
TCCGCAC11750.016.44100664
CCGCACC11900.015.9437365
CGCACCT12150.015.33160866
TAGGTAG13350.015.247455
GGCTCGG7200.013.89607055
TAGTCAG12950.013.5844629
GGTAGTC13700.012.5904057
GTATATA54350.012.5052741
CTATGCA21850.011.997879
CTAATAC29900.011.8845983
CTAGACT10500.011.8287734
GCTCGGA9050.011.8175536
GTATAAT36950.011.6713771
GTATTAT48650.011.5592931
ACATAGG14350.011.54133531
GGGACTG10500.011.50126231
GTATAGA26350.011.3910871
GTATAAC23600.011.2565481
TCCTTGC28150.011.1513948
Produced by FastQC (version 0.11.2)