FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186246
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18510.9938468477175348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGC350.001345889839.42273711
CCCTACA400.002592649534.4948962
AGCCGGA605.4096547E-428.74574710
CTACACC500.007724116627.5959154
TACGCGT1359.3605195E-925.54490969
GAATGGG851.4384068E-424.3493371
GTATTAG750.001988497322.9965951
TCCTATC750.001988497322.9965953
AGTGCAG750.001988497322.99659510
GTACTAT750.001988497322.9965951
CAGTCTC750.001988497322.9965959
GTAGTCA750.001988497322.9965958
ACGCGTG750.001991608922.99041769
TTACGCG1605.662514E-821.5593168
ACCGTCC800.002892889521.559318
CTATATT1004.3203344E-420.6969364
TTTCCGC850.00411094420.29111510
ATCACTG850.00411094420.2911155
TCTAACA850.00411094420.2911152
GCCGTTT950.00780499518.1600926
Produced by FastQC (version 0.11.2)